ank2 (mouse, icc Search Results


ankyrin b polyclonal antibody  (Bioss)
90
Bioss ankyrin b polyclonal antibody
Ankyrin B Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ank2 (mouse, icc  (Thermo Fisher)
90
Thermo Fisher ank2 (mouse, icc
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Ank2 (Mouse, Icc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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collagen 7 polyclonal antibody  (Bioss)
92
Bioss collagen 7 polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Collagen 7 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen 7 polyclonal antibody - by Bioz Stars, 2026-03
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prrsv m protein polyclonal antibody  (Bioss)
92
Bioss prrsv m protein polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Prrsv M Protein Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
prrsv m protein polyclonal antibody - by Bioz Stars, 2026-03
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8-ohdg polyclonal antibody  (Bioss)
96
Bioss 8-ohdg polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
8 Ohdg Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pan cytokeratin polyclonal antibody  (Bioss)
94
Bioss pan cytokeratin polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Pan Cytokeratin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pan cytokeratin polyclonal antibody - by Bioz Stars, 2026-03
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cd16 polyclonal antibody  (Bioss)
94
Bioss cd16 polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Cd16 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd16 polyclonal antibody - by Bioz Stars, 2026-03
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acetyl coa carboxylase antibody  (Bioss)
94
Bioss acetyl coa carboxylase antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Acetyl Coa Carboxylase Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdc2/cdk1 polyclonal antibody  (Bioss)
94
Bioss cdc2/cdk1 polyclonal antibody
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Cdc2/Cdk1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α-tubulin (mouse, ib  (Millipore)
90
Millipore α-tubulin (mouse, ib
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
α Tubulin (Mouse, Ib, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scn2a (mouse, ib  (NeuroMab)
90
NeuroMab scn2a (mouse, ib
A. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the ankyrin/spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and <t>Scn2a</t> were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands respectively. Transparent overlays mark the location of each Kcc2 complex.
Scn2a (Mouse, Ib, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ank3 (rabbit  (Synaptic Systems)
90
Synaptic Systems ank3 (rabbit
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, <t>ANK3,</t> CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.
Ank3 (Rabbit, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, Ank2, Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).

Journal: bioRxiv

Article Title: Isolation and characterization of multi-protein complexes enriched in the K-Cl co-transporter 2 from brain plasma membranes

doi: 10.1101/2020.04.30.071076

Figure Lengend Snippet: A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, Ank2, Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (Icc): Ank2 (mouse, Icc, Invitrogen 33-3700), Ank2 (rabbit, IB, Bioss BS-6967R-TR), Cntn1 (rabbit, IB), Abcam Ab66265), Itpr1 (rabbit, IB/Icc, Alomone Acc-019), Kcc2 (mouse, IP/Icc, Neuromab 75-013), Kcc2 (rabbit, IB/Icc, Millipore 07-432), Scn2a (mouse, Neuromab 75-024), Scn2a (rabbit, IB, Alomone ASC-002), Sptan1 (mouse, Icc, Abcam Ab11755), Sptan1 (rabbit, IB, Cell Signaling 21225), Sptbn1 (rabbit, IB/Icc, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Cell Culture, Infection

A. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the ankyrin/spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and Scn2a were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands respectively. Transparent overlays mark the location of each Kcc2 complex.

Journal: bioRxiv

Article Title: Isolation and characterization of multi-protein complexes enriched in the K-Cl co-transporter 2 from brain plasma membranes

doi: 10.1101/2020.04.30.071076

Figure Lengend Snippet: A. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the ankyrin/spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and Scn2a were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands respectively. Transparent overlays mark the location of each Kcc2 complex.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (Icc): Ank2 (mouse, Icc, Invitrogen 33-3700), Ank2 (rabbit, IB, Bioss BS-6967R-TR), Cntn1 (rabbit, IB), Abcam Ab66265), Itpr1 (rabbit, IB/Icc, Alomone Acc-019), Kcc2 (mouse, IP/Icc, Neuromab 75-013), Kcc2 (rabbit, IB/Icc, Millipore 07-432), Scn2a (mouse, Neuromab 75-024), Scn2a (rabbit, IB, Alomone ASC-002), Sptan1 (mouse, Icc, Abcam Ab11755), Sptan1 (rabbit, IB, Cell Signaling 21225), Sptbn1 (rabbit, IB/Icc, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Isolation, Immunoprecipitation

A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Isolation

A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Cell Culture, Infection

A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: SDS Page, Expressing