Bioss ankyrin b polyclonal antibody
Ankyrin B Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews

ankyrin b polyclonal antibody - by Bioz Stars, 2026-09

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8-ohdg polyclonal antibody (Bioss)

Bioss 8-ohdg polyclonal antibody
8 Ohdg Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen 7 polyclonal antibody (Bioss)

Bioss collagen 7 polyclonal antibody
Collagen 7 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrsv m protein polyclonal antibody (Bioss)

Bioss prrsv m protein polyclonal antibody
Prrsv M Protein Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pan cytokeratin polyclonal antibody (Bioss)

Bioss pan cytokeratin polyclonal antibody
Pan Cytokeratin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd16 polyclonal antibody (Bioss)

Bioss cd16 polyclonal antibody
Cd16 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acetyl coa carboxylase antibody (Bioss)

Bioss acetyl coa carboxylase antibody
Acetyl Coa Carboxylase Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdc2/cdk1 polyclonal antibody (Bioss)

Bioss cdc2/cdk1 polyclonal antibody
Cdc2/Cdk1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kcc2 (NeuroMab)

NeuroMab kcc2
$A. Differential centrifugation of homogenized forebrain from 8-12-week-old mice was used to fractionate cellular organelle and obtain an enriched plasma membrane fraction. B. Western blots of the organelle fractions were used to measure the abundance of organelle markers; HSP90 (cytosol), HSP60 (mitochondria), calreticulin (ER/Golgi), n-Cadherin (n-Cadh) (plasma membrane). <t>KCC2</t> abundance was also measured to confirm KCC2 enrichment in the plasma membrane fraction. C. Blue Native PAGE (BN-PAGE) was carried out on plasma membrane lysates, and immunoprecipitated KCC2 to resolve the native protein complexes that contain KCC2. These were correlated with protein bands observed by Coomassie staining. D. Detected peptides were mapped to the KCC2 reference sequence to determine KCC2 sequence coverage obtained from LC-MS/MS of BN-PAGE protein bands produced following KCC2 IP. Bar charts of the KCC2 sequence coverage expressed as a percentage of the full sequence and by total KCC2 peptides detected (n=4).$
Kcc2, supplied by NeuroMab, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ank3 (NeuroMab)

NeuroMab ank3
$A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, <t>ANK3,</t> CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.$
Ank3, supplied by NeuroMab, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scn2a (NeuroMab)

NeuroMab scn2a
$A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.$
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Image Search Results

$A. Differential centrifugation of homogenized forebrain from 8-12-week-old mice was used to fractionate cellular organelle and obtain an enriched plasma membrane fraction. B. Western blots of the organelle fractions were used to measure the abundance of organelle markers; HSP90 (cytosol), HSP60 (mitochondria), calreticulin (ER/Golgi), n-Cadherin (n-Cadh) (plasma membrane). KCC2 abundance was also measured to confirm KCC2 enrichment in the plasma membrane fraction. C. Blue Native PAGE (BN-PAGE) was carried out on plasma membrane lysates, and immunoprecipitated KCC2 to resolve the native protein complexes that contain KCC2. These were correlated with protein bands observed by Coomassie staining. D. Detected peptides were mapped to the KCC2 reference sequence to determine KCC2 sequence coverage obtained from LC-MS/MS of BN-PAGE protein bands produced following KCC2 IP. Bar charts of the KCC2 sequence coverage expressed as a percentage of the full sequence and by total KCC2 peptides detected (n=4).$

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Differential centrifugation of homogenized forebrain from 8-12-week-old mice was used to fractionate cellular organelle and obtain an enriched plasma membrane fraction. B. Western blots of the organelle fractions were used to measure the abundance of organelle markers; HSP90 (cytosol), HSP60 (mitochondria), calreticulin (ER/Golgi), n-Cadherin (n-Cadh) (plasma membrane). KCC2 abundance was also measured to confirm KCC2 enrichment in the plasma membrane fraction. C. Blue Native PAGE (BN-PAGE) was carried out on plasma membrane lysates, and immunoprecipitated KCC2 to resolve the native protein complexes that contain KCC2. These were correlated with protein bands observed by Coomassie staining. D. Detected peptides were mapped to the KCC2 reference sequence to determine KCC2 sequence coverage obtained from LC-MS/MS of BN-PAGE protein bands produced following KCC2 IP. Bar charts of the KCC2 sequence coverage expressed as a percentage of the full sequence and by total KCC2 peptides detected (n=4).

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Centrifugation, Clinical Proteomics, Membrane, Western Blot, Blue Native PAGE, Immunoprecipitation, Staining, Sequencing, Liquid Chromatography with Mass Spectroscopy, Produced

A. Pie charts showing the average number of total KCC2 peptides detected by LC-MS/MS relative to the average number of total peptides detected for all other proteins in each molecular weight complex. B. Venn diagrams showing the number and overlap of KCC2-associated proteins identified in each of 4 biological replicates in each molecular weight complex. C. Venn diagram showing the overlap in associated proteins detected in at least 3 of the 4 replicates for each molecular weight complex. D. PCA analysis of each biological replicate for each molecular weight complex based on their protein composition.

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Pie charts showing the average number of total KCC2 peptides detected by LC-MS/MS relative to the average number of total peptides detected for all other proteins in each molecular weight complex. B. Venn diagrams showing the number and overlap of KCC2-associated proteins identified in each of 4 biological replicates in each molecular weight complex. C. Venn diagram showing the overlap in associated proteins detected in at least 3 of the 4 replicates for each molecular weight complex. D. PCA analysis of each biological replicate for each molecular weight complex based on their protein composition.

Techniques: Liquid Chromatography with Mass Spectroscopy, Molecular Weight

A. A network diagram including the 150 most abundant proteins from the 600 kDa complex based on total peptide counts. Known interactions were obtained using stringent high confidence, direct experimental association parameters from StringDB. These were used to construct a network diagram of protein nodes and arrows to indicate known interactions. The interactions for each protein with KCC2 were included as discovered here. An overlay of gene ontology (GO) terms was used to provide protein classification information. B. A network diagram for the most abundant 150 proteins in the 800 kDa complex, analyzed as described above (n=4).

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. A network diagram including the 150 most abundant proteins from the 600 kDa complex based on total peptide counts. Known interactions were obtained using stringent high confidence, direct experimental association parameters from StringDB. These were used to construct a network diagram of protein nodes and arrows to indicate known interactions. The interactions for each protein with KCC2 were included as discovered here. An overlay of gene ontology (GO) terms was used to provide protein classification information. B. A network diagram for the most abundant 150 proteins in the 800 kDa complex, analyzed as described above (n=4).

Techniques: Construct

A. A network diagram of KCC2-associated proteins from the 600 kDa complex, that are also the protein products of genes associated with ASD or epilepsy. The known protein associations were sourced from StringDB and applied with an overlay of ASD/Epi risk genes from the SFARI, and EpilepsyGene databases. The colors indicate whether the proteins are implicated in ASD or epilepsy alone, or both. B. The same analysis was carried out for the 800 kDa protein complex (n=4).

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. A network diagram of KCC2-associated proteins from the 600 kDa complex, that are also the protein products of genes associated with ASD or epilepsy. The known protein associations were sourced from StringDB and applied with an overlay of ASD/Epi risk genes from the SFARI, and EpilepsyGene databases. The colors indicate whether the proteins are implicated in ASD or epilepsy alone, or both. B. The same analysis was carried out for the 800 kDa protein complex (n=4).

Techniques:

$A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.$

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.

Techniques: Isolation, Clinical Proteomics, Membrane

A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Techniques: Cell Culture, Infection

$A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.$

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.

Techniques: Clinical Proteomics, Membrane, SDS Page, Expressing

$A. KCC2 was isolated from plasma membrane fractions of mouse forebrain and resolved by SDS-PAGE. B. Bar graphs of high confidence phosphorylated peptides relative to total detected peptides in wild type and FMR1 KO mice. For bars where phosphorylation was detected, A scores are superimposed to express phosphoryation confidence levels. C. Diagram of high confidence KCC2 phosphorylation site positions.$

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. KCC2 was isolated from plasma membrane fractions of mouse forebrain and resolved by SDS-PAGE. B. Bar graphs of high confidence phosphorylated peptides relative to total detected peptides in wild type and FMR1 KO mice. For bars where phosphorylation was detected, A scores are superimposed to express phosphoryation confidence levels. C. Diagram of high confidence KCC2 phosphorylation site positions.

Techniques: Isolation, Clinical Proteomics, Membrane, SDS Page, Phospho-proteomics

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Isolation, Clinical Proteomics, Membrane

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Cell Culture, Infection

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Clinical Proteomics, Membrane, SDS Page, Expressing

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Isolation, Clinical Proteomics, Membrane

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Cell Culture, Infection

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Techniques: Clinical Proteomics, Membrane, SDS Page, Expressing

ank2 (mouse, icc Search Results

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